【Abstract】 Objective To determine the lethal feeding period (LFP) of 0.0025% Chlorophacinone baits in Meriones unguiculatus. Methods A LFP?resistance?based detection method developed by the EPPO (European and Mediterranean Plant Protection Organization) was adopted. Results The minimum lethal dose was 6.3510 mg/kg for M. unguiculatus, the highest survival dose 13.0230 mg/kg. Statistically significant differences were observed in the feeding period and its corresponding mortalities  between  male  and  female  groups (P＝0.036),  the  Probit  model (feeding  period  versus  mortality)  being  Probit (p)＝-2.651 95＋0.798 17x (P＝0.901),  and Probit(p)＝-5.620 58＋2.160 45x (P＝0.999), respectively. The LFP50, LFP98, LFP99 and  their  95%  confidence  limits(CI)  were  3.322 53 d(2.740 04-3.952 39),  5.895 58 d(4.939 49-8.323 31),  and  6.237 11 d (5.184 50-8.950 40) in  the  female  M. unguiculatus;  and  2.601 59 d (2.210 62-2.989 34), 3.552 20 d (3.115 84-5.024 47)  and  3.678 38 d (3.204 01-5.326 58) in the male  M. unguiculatus.  Conclusion In  LFP  resistance  detection  using  0.0025%  Chlorophacinone  baits  in M. unguiculatus, the 95%CI upper limit of LFP98, an integer number of days when applied as the drug?resistant testing standards, was 8 d for female rats and 5 d for males that survived in the feeding period; on the other hand, the 95%CI upper limit of LFP99, as an alternative, was 9 d for surviving female rats and 5 d for surviving males in the feeding period.

【Abstract】 Objective To study the anti?fertility effect of gossypol to Lasiopodomys brandtii. Methods The effect of gossypol to sperm quality, testis and epididymis tissues of L.brandtii was tested with one?off quantitative gastric gavages. Results Gossypol did harm to spermatids, spermatocytes and testis tissue lesions. Testis and epididymis tissues coefficient decreased obviously at the dosage of 20-100 mg/kg, and sperm concentration reduced and sperm malformation increased at 20 mg/kg. It leaded to the death of L.brandtii when the dosage exceeded 60 mg/kg. Conclusion The gossypol had significant anti?fertility effect on male L.brandtii at the concentration of 20-100 mg/kg, but it was lethal at high concentrtion.

Objective To study the killing efficiency of one million MLD/ml Type-C Botulinum lyophilized preparation to Ochotona curzoniae. Methods Five plots was selected to test and each area was 3 hm ². 0.1% wheat baits were put in two plots randomly, other two-plot for 0.15% and the last one for aqueous agent. Besides, the control was 0.25 hm ². Results The density of O.curzoniae was 1071.4 holes/hm ² before treatment. The killing efficiency of 0.1% wheat baits and 0.15% wheat baits were 96.7% and 97.4% after treatment for six days, respectively. However, the killing efficiency was also 97.7% in the control plots. It showed that there was no significant difference among them. During the test, there were no no-target animals to be poisoned in test plots and within 500 m of it. It indicated that Type-C Botulinum was safe to some degree, but the safety of the chemicals was still further validated in the long run. Conclusion There is no significant difference between botulinum lyophilized preparation and the same titer aqueous botulinum.

Objective To test the resistance to warfarin by the striped field mouse ( Apodemus agrarius) captured from Shunyi district of Beijing. Methods 33 mice were given the bait which contained 0.02% warfarin in 6 consecutive days with no-choice way in laboratory. Results The warfarin bait caused 63.64% mortality, the average ingestion of poison of dead mice was (76.91±34.76)mg/kg and the average lethal time was (7.23±2.51)d. The resistant percentage was 36.36% and the average ingestion of poison of survival individuals was (106.79±26.68)mg/kg. Conclusion (1) The striped field mouse from Shunyi district has become resistant to warfarin after 20 years’ application of the second generation of anticoagulant rodenticides, which resulted in the resistant group;(2) The female mouse has broader sensitivity range than the male one; the juvenile and old group have higher resistance than adult and sub-adult ones. It is suggested that the no-choice of six-day feeding resistance test program is suitable for monitoring the resistance to anticoagulant rodenticides by rodents and integrative methods are necessary to reduce resistance of rodents and to prolong the time of using anticoagulant rodenticides.

Objective To determine acute toxicity of chlorophacine-sodium in Brandti's voles( Lasiopodomys brandtii).Methods Brandti's voles were treated with chlorophacine-sodium via gastric tube at concentrations of eight gradients based on its median lethal dose LD ₅₀ by modified's Krber's method.Results LD ₅₀. of chlorophacine-sodium in Brandti's voles was(5.913 mg/kg) with a standard error of 0.082 mg/kg and 95% confidence limit of 5.913±2.172 mg/kg,without significant difference between male and female voles by χ ² test( P>0.05).Conclusion Acute toxicity of chlorophacine-sodium in Brandti's voles reached the highest level according to classification standard for toxic chemicals in mice and could be used as an alternative rodenticide with chronic-toxicity in rat control.